Update on new E. coli outbreak from Robert Koch Institute

The Robert Koch Institute have carried out microbiology analysis on the new E. coli strain. It appears to carry a particularly nasty cocktail of virulence genes (Shigatoxin 2 + enteroaggerative virulence factors). Details from the RKI leaflet are:

EHEC O104:H4

The current EHEC O104:H4 , causing a severe outbreak in germany ( May 2011), is microbiologically characterized as follows (1) (27.5.2011; updated 01.6.2011) :

  • Shigatoxin 1:    – (negative)
  • Shigatoxin 2 (vtx2a) : + (positive)
  • Intimin (eae) :    – (negative)
  • Enterohemolysin : – (negative)

EaggEC virulence plasmid:

  • aatA-PCR: + (positive) (ABC-transporter protein gene)
  • aggR-PCR: + (positive) (master regulator gene of Vir-plasmid genes)
  • aap-PCR: + (positive) (secreted protein dispersin gene)
  • aggA-PCR: + (positive) (AAF/I-fimbral subunit-gene)
  • aggC-PCR: + (positive) (AAF/I-fimbral operon-gene)

MLST Sequence Type:
ST678 (adk 6, fumC6, gyrB 5, icd 136, mdh 9, purA 7, recA 7). (**)
(**) MLST : kindly provided by Prof. Dr. H. Karch; Konsiliarlaboratorium für Hämolytisch-Urämisches Syndrom (HUS) Institut für Hygiene am Universitätsklinikum Münster Robert-Koch-Str. 41, 48149 Münster .

  • Ampicillin    R
  • Amoxicillin/Clavulanic acid    R
  • Piperacillin/Sulbactam    R
  • Piperacillin/Tazobactam    R*
  • Cefuroxim    R
  • Cefuroxim-Axetil    R
  • Cefoxitin    R
  • Cefotaxim    R
  • Cetfazidim    R
  • Cefpodoxim    R
  • Imipenem    S
  • Meropenem    S
  • Amikacin    S
  • Gentamicin    S
  • Kanamycin    S
  • Tobramycin    S
  • Streptomycin    R
  • Nalidixinsäure    R
  • Ciprofloxacin    S
  • Norfloxacin    S
  • Tetracyclin    R
  • Nitrofurantoin    S
  • Trimethoprim/Sulfamethoxazol    R
  • Chloramphenicol    S
  • Fosfomycin    S

* defined as resistent (AES VITEK)

  • ESBL: CTX-M-15 + (positive)
  • Other ß-Lactamases: TEM-1 + (positive)

1) Source: Nationales Referenzzentrum für Salmonellen und andere bakterielle Enteritiserreger; Robert Koch-Institut; Abteilung 1 Infektionskrankheiten

Thanks to Jon Hobman for forwarding the link.

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